References, Application Notes and Product Sheets

Application notes

• Absolute quantitation of protein biomarkers in FFPE tissue: Case study: Determining Her2 expression in invasive breast cancer using a Liquid Tissue® Multiple Reaction Monitoring (MRM) assay (PDF)
• Discovery of protein biomarkers in endometrial cancer (PDF)

• Identification of Prognostic Biomarkers of Metastatic Lung Cancer Survival and their use to predict survival ( PDF)

• A Molecular Portrait of Head and Neck Cancer Progression (PDF)

Publications

• Proteomic Analysis of Laser-Microdissected Paraffin-Embedded Tissues: (1) Stage-Related Protein Candidates Upon Non-Metastatic Lung Adenocarcinom. Takashi Kawamura, et al (2009) J. Proteomics, Vol. 7, Issue 6, 18 April, 2010, Pages 1089-1099 http://dx.doi.org/10.1016/j.jprot.2009.11.011
• Proteomic Analysis of Laser-Microdissected Paraffin-Embedded Tissues: (2) MRM Assay for Stage-Related Proteins Upon Non-Metastatic Lung Adenocarcinom.Toshihide Nishimura et al (2009) J. Proteomics, Vol. 7, Issue 6, 18 April, 2010,
  Pages 1100-1110 http://dx.doi.org/10.1016/j.jprot.2009.11.010
• LC/MS-Based Quantitative Proteomic Analysis of Paraffin-Embedded Archival Melanomas Reveals Potential Proteomic Biomarkers Associated with Metastasis. Sharon K. Huang, Marlene M. Darfler, Michael B. Nicholl, Jinsam You, Kerry G. Bemis, Tony J. Tegeler, Mu Wang, Jean-Pierre Wery, Kelly K. Chong, Linhda Nguyen, Richard A. Scolyer, Dave S. B. Hoon (2009) Public Library of Science Online, PLoS ONE 4(2): e4430. doi:10.1371/journal.pone.0004430
• Application of a Global Proteomic Approach to Archival Precursor Lesions: Deleted in Malignant Brain Tumors 1 and Tissue Transglutaminase 2 Are Upregulated in Pancreatic Cancer Precursors. Wang Cheung, Marlene M. Darfler, Hector Alvarez a Brian L. Hood, Thomas P. Conrads, Nils Habbe, David B. Krizma, Jan Mollenhauer, Georg Feldmann, Anirban Maitra (2008) Pancreatology 2008;8:608–616
• Quantitative Proteomic Analysis of Formalin Fixed Paraffin Embedded Oral HPV Lesions from HIV Patients. Mohit Raja Jain, Tong Liu, Jun Hu, Marlene Darfler, Valerie Fitzhugh, Joseph Rinaggio, and Hong Li (2008) The Open Proteomics Journal 1, 40-45, June 15, (abstract)
• Proteomic Analysis of Laser-Captured Paraffin-Embedded Tissues: A Molecular Portrait of Head and Neck Cancer Progression. Vyomesh Patel, Brian L. Hood, Alfredo A. Molinolo, Norman H. Lee, Thomas P. Conrads, John C. Braisted, David B. Krizman, Timothy D. Veenstra and J. Silvio Gutkind (2008) Clinical Cancer Research 14, 1002-1014, February 15 (abstract) (white paper summary)
• Proteomic Analysis of Formalin-Fixed Prostate Cancer Tissue. Hood, B.L., Darfler, M.M., Guiel, T.G., Furusato, B., Lucas, D.A., Ringeisen, B.R., Sesterhenn, I.A., Conrads, T.P., Veenstra, T.D. and Krizman, D. B. (2005) Mol. Cell. Proteomics 4, 1741-1753 ( article)
• Liquid Tissue: Proteomic profiling of formalin-fixed tissues. Prieto, D.A., Hood, B.L., Darfler, M.M., Guiel, T.G., Lucas, D.A., Conrads, T.P., Veenstra, T.D. and Krizman, D. B. (2005) Biotechniques 38, S32 - S35) ( article)

Product Literature

• Liquid Tissue® MS Protein Prep Kit—Discovery, validation and measurement of proteins in FFPE tissue (PDF)
• DIRECTOR® Laser Microdissection Slides—Direct non-contact microdissection without plastic film or sticky caps (PDF

Microdissection

Laser microdissection - essential for tissue proteomic analysis. Download the video showing the principles of Fast Forward Microdissection Download 3Mb .wmv file