The
patent pending process used in the Liquid Tissue® MS
Protein Prep Kit enables mass spectrometry-based discovery and
measurement
of protein biomarkers in archival tissue. It has been used to
identify over 2,000 proteins extracted from FFPE samples using
LC-MS/MS mass spectrometry*.
A protocol is available to detect differential protein expression
using iTRAQ™** labeling.
Download it here.
A standard preparation uses a lot less tissue than any other
published methods. The Liquid Tissue® MS Protein Prep Kit
is optimized for processing approximately 30,000 cells, the equivalent
of a 2 x 4 mm region from a 10 µm thick section of FFPE
tissue, and will yield 5-10 µg of total protein.
Make sure you are using
the most recent revision of
the instruction manual.
ORDERING INFORMATION
Catalog Number
Product Description
10001-010
Liquid Tissue® MS Protein Prep Kit, 4 preparations
10001-023
Liquid Tissue® MS Protein Prep Kit, 10 preparations
10001-053
Liquid Tissue® MS Protein Prep Kit,
50 preparations
Inquire for price and availability for the larger package sizes.
Samples
processed using the Liquid Tissue® MS Protein Prep Kit are
not suitable for analysis using gel electrophoresis or for printing
reverse-phase protein arrays.
DIRECTOR™ Laser
Microdissection Slides—Direct non-contact
microdissection without plastic film or sticky
caps
Fast Forward Microdissection
- for the Leica LMD and the P.A.L.M. Zeiss systems
Watch the videos
showing the principles and the slides in use
The
unique DIRECTOR slides feature a proprietary energy transfer
coating that replaces plastic films or adhesives for direct non-contact
microdissection. This makes possible fast and precise harvesting
of even the most specific cellular features, from
fine,
subcellular features to larger regions of tissue.
DIRECTOR slides are compatible with commonly used gene and protein
expression analysis methods, and work with tissues or cells,
whether fresh, frozen or fixed (inc. standard formalin
fixed paraffin embedded (FFPE) tissue).
The slides are inert to a wide range of chemical
and thermal conditions, and can be autoclaved, and are stable
in
organic solvents such as xylenes. DIRECTOR slides will not epifluoresce,
making them ideally suited for applications requiring fluorescent
stains, as well as those applications requiring DIC or polarized
light. Tissue sections will adhere to the DIRECTOR
slides just as well as to any standard pathology glass slides,
without pretreatment.
The DIRECTOR slides are based on Laser Induced Forward Transfer
(LIFT), a non-contact microdissection technology utilizing a
thin energy
transfer coating. Laser energy is transferred to the transfer
coating, the coating is vaporized and the selected feature instantly
shot
into
the collection tube. You collect exactly what you see in the
microscope. The energy transfer coating absorbs the laser energy,
so the biomolecules
in the sample are not affected. Since the coating is completely
vaporized there is absolutely no contamination of your sample.
ORDERING INFORMATION
Catalog Number
Product Description
50001-018
DIRECTOR™
Laser Microdissection Slides, 10 slides
50001-024
DIRECTOR™
Laser Microdissection Slides, 50 slides
Volume
discounts available.
ProteoSOL™
Tissue Extraction System
Available from KPL,
Kirkegaard & Perry Laboratories, the ProteoSOLTM
Tissue Extraction Systemis based on the Liquid Tissue®
ID Protein Prep Kit. It is designed for the extraction of proteins
directly from formalin fixed paraffin-embedded (FFPE) tissue samples.
The system enables immunodetection of proteins in a membrane-based
assay. Samples processed with this system can be arrayed and detected
on a membrane using high-throughput detection methods. Immunodetection
with specific antibodies allows the direct comparison of a specific
protein across multiple samples in a single array.
*Please note that samples processed using
the ProteoSOL Tissue Extraction System are not suitable for analysis
using mass spectrometry or gel electrophoresis.
, *Thermo
Finnigan Proteome X LTQ Mass Spectrometer .** iTRAQ
is a trademark of Applied Biosystems.
Call us before you start! Our
scientists have extensive experience in pathology,
protein expression, microdissection, mass spectrometry
and immunodetection, and can provide advice and suggestions.
TO ORDER
Orders can be placed by calling 1.301.977.3654,
American Eastern Time, 9AM to 5PM. A written order
confirmation must be faxed to
1.301.926.9283. We accept VISA and MasterCard, and
purchase orders from the United States and Canada.
While we are building our distributor network, international
orders are shipped only as credit card orders or
after
receipt of a wire transfer against a pro forma invoice.
Please inquire for quotations and shipping details to other parts of the world.
news sidebar
News & Events
ASMS,
Denver, June 1-5. (link)
Booth 21. Contact us about our tissue proteomic luncheon
seminar on Wednesday, June 4.
American Association of Cancer Research, San
Diego, April 12-16. (link)
Booth 2428. See three posters using our technology; discovery
of endometrial cancer markers (abstract),
metastasis marker in melanoma (abstract), protein biomarkers relating to metastasis and disease
progression in lung cancer (abstract).
A Molecular Portrait of Head and Neck Cancer Progression,
Clin. Cancer Res., Feb. 15, 2008 (abstract)
Expression Pathology Aids Research in Identifying Biomarkers
of Lung Cancer Metastasis and Survival, Feb. 19, 2008 (article
and press release)
Expression Pathology Obtains Maryland Funding to Develop
Breast Cancer Test, Feb. 6, 2008(press
release)
Lecia Microsystems to sell DIRECTOR laser microdissection
slides, Sept. 10, 2007 (press
release)
The
patent pending process used in the Liquid Tissue® MS
Protein Prep Kit enables mass spectrometry-based discovery and
measurement
of protein biomarkers in archival tissue. It has been used to
identify over 2,000 proteins extracted from FFPE samples using
LC-MS/MS mass spectrometry*. 